determination of lethal dose of plant extract

Phytochemical screening, proximate analysis, median lethal ...

Administration of A. precatorius seed extracts: The method of Yamba et al. (2007) was used for the selection of doses used in hematological and serum biochemistry studies. The selected doses were within the range between tenth and one hundredth of the estimated median lethal dose of aqueous (187.5 mg/Kg) and ethyl acetate (175 mg/Kg) extract.

Oral Administration of Aqueous Leaf Extract of Ocimum ...

The median lethal dose (LD 50) of the plant extract was determined by method of Lorke (1983)[12] using 35 rats weighing between 60 – 100g. In the first phase, 15 rats were divided into 3 groups of 5 rats each and were treated with the aqueous leaf extract of the plant at dosages of 1000, 1500 and 2000mg/kg body weight intra-peritoneally.

ACUTE TOXICITY AND DOSE RESPONSE STUDIES OF …

histological examination after 28 days of administration of plant extracts. The lethal dose (LD50) was beyond 5000 mg/kg body weight for both extracts investigated as no signs of toxicity were observed even at larger doses. Dose response studies show a dose dependent increase (P<0.05) in the activities

A PILOT STUDY ON THE DETERMINATION OF ANTIOXIDANT ...

A PILOT STUDY ON THE DETERMINATION OF ANTIOXIDANT POTENTIAL AND LETHAL ... the lethal dosage of fruit extract of . Terminalia chebula, a traditional medicinal plant widely used for various ailments. ... The reducing power of the plant extract was determined by the method of Oyaizu et al[18] Varying concentrations of the extracts

Phytochemical screening, proximate analysis, median lethal ...

this, phytochemical screening, proximate analysis, median lethal dose (LD 50), hematological and biochemical effects of extracts of A. precatorius seed was studied in Mus musculus. Materials and methods: Nineteen (19) mice were used for the study. Four (4) mice were used for determination of median lethal dose of the aqueous and ethyl

Effect of the ethanolic leaf extract of Moringa oleifera ...

A dark brownish extract, pH 6.5, having aromatic medicinal plants odour, coarse texture with a yield of 10.06% (w/w) was obtained from the ethanolic leaf extraction. 3.2. Acute Toxicity Test in Mice Acute toxicity test was done to determine the median lethal dose of the ethanolic leaf extract …

DETERMINATION OF MEDIAN LETHAL DOSE OF …

Packaging of Extract/Weight A spatula was used to collect the extract into a 20ml weighed beaker (pyrex), covered with a foil paper and preserved at normal room temperature. The weight of the extract was determined using a chemical balance. Determination of the Median Lethal Dose The median lethal dose of the ethanolic extract of

In vitro neutralization of Naja nigricollis venom by stem ...

2.7.1 Acute Toxicity Test on Extract The determination of the median lethal dose (LD 50) of the stem-bark methanol extract of C. africana was carried out in mice by the method described by Lorke [29] (1983). In the initial phase, 9 mice were randomly divided into three groups of three mice each.

Assessment of Liver Histomorphology and Curative Effect of ...

Table 2: Lethal Dose determination using proposed new method. Administration of carbon tetrachloride and extract: The carbon tetrachloride and the extract was administered to the group of rats involved using 0.5 ml/kg of body weight by oral route along with 0.5 mg/kg equal volume of olive oil which will serve as its mediator for 14 days.

Cumin: Uses, Side Effects, Interactions, Dosage, and Warning

Ozbek H, Ozturk M, Ozturk A, and et al. Determination of lethal doses of volatile and fixed oils of several plants. Eastern Journal of Medicine (EAST J MED) 2004;9(1):4-6.

Oral acute toxicity (LD50) study of different solvent ...

Table 4: Results of median lethal dose determination of A. precatorius solvent extracts Solvent extracts Log LD50 LD50 (mg/kg) Aqueous 3.370 2, 345 70% Methanol 3.596 3,942 Acetone 2.272 187 Petroleum ether 2.610 407 Effects of acute oral administration of A. precatorius extracts on body weights and organ weights

Effects of aqueous leaves extract of Ocimum gratissimum on ...

The lethal doses (LD 50) of the plant extract was determined by method of Lorke (1983) using 13 rats. In the first phase, rats were divided into 3 groups of 3 rats each and were treated with the aqueous extract of the plant at doses of 10, 100 and 1000 mg/kg bodyweight intraperitoneal. They were observed for 24 h for signs

Acute Toxicity (Lethal Dose 50 Calculation) of Herbal Drug ...

In this study, different doses (10, 100, 285, 500, 1000, 5000 and 10,000 mg/kg) of the extract was administered orally to the different groups of rats and mice. Signs of toxicity and possible death of animals were monitored for 24 hrs to calculate the median lethal dose (LD50) of somina.

ISSN 2230 ACTIVE PRINCIPLES AND MEDIAN LETHAL DOSE …

KEYWORDS: Active principles, Curcuma longa (Haldi), Median lethal dose, Phytochemical study, Toxicity study. INTRODUCTION Extractability of any plant-drug serves as a tool for quality control, and provides an idea regarding the amount of extract present in a definite quantity of drug.

Department of Pharmacognosy and Drug Development, ABU ...

ethanol extract of C. africana was carried out to identify the chemical constituents using standard analytical techniques as described by Evans (2009). 2.6 Determination of Media Lethal Dose (LD 50) The media lethal dose (LD 50) of the ethanolic stem bark …

Sage Uses, Benefits & Dosage - Drugs.com Herbal Database

Dec 01, 2017· Aherne 2007 In rats, the median lethal dose (LD 50) of a methanolic extract of sage leaves was calculated to be 4,000 mg/kg when injected intraperitoneally.Eidi 2006, Ghorbani 2017 The LD 50 of orally administered S. officinalis oil is reported to be 2.6 g/kg.

Determining LD50 for a plant extract - ResearchGate

Determining LD50 for a plant extract. What is the basis for selecting the minimal dose of plant extract for calculating lethal dose for an animal. ... LD50 is an important factor for determination ...

Yohimbe Dosage Guide, Timing and Dangers of Overdose

Yohimbe dosage recommendations vary based on the goal of the supplement user. Determining how much you should take daily depends on your situation and the way you intend to use it. This herbal extract is taken orally as a tea or a pill. It is also available as a liquid tincture or a bulk powder.

Acute and Subacute Toxicities of African Medicinal Plants ...

Some acute toxicity tests (such as the "classical" LD 50 test) are designed to determine the mean lethal dose of the test substance. The median lethal dose (or LD 50) is defined as the dose of a test substance that is lethal for 50% of the animals in a dose group. 5.2. The Acute and Subacute Effects of Medicinal Plants

The activity of alcoholic extract of Garlic on the growth ...

determine the median lethal dose ( LD 50) of the extract in lab. mice. The Garlic was extracted by ethyl alcohol 95%, the ratio of ethanolic extraction amounted 44% of the weight of dry substance. Graduated concentration were prepared from alcoholic extract of Garlic from 10-100 mg/ml.

determination of lethal dose of plant extract

Analysis of the procedure for determining the LD50. Bioactivity, bioassay, median lethal dose (LD50), quantal analysis, statistical quantal, Probit, ... determination of ld50 (1) plant extract LD50 testing (1)

Phytochemical Screening and Acute Toxicity of Aqueous ...

(median lethal dose) was used the methodology of according with Acute Toxic Class Method (OECD 2001) for acute toxicity test of single dose (Guideline 423). In this acute toxicity study, the initial dose of 5,000 mg/kg bw was used due to known information about toxicity of the plant (Abdel-Hameed et al. 2013). The next tested

Acute Oral Toxicity and Brine Shrimp Lethality of Methanol ...

Purpose: To determine, in Sprague Dawley rats, the toxicity profile of the methanol extract of Mentha spicata, a plant used in folklore medicine for the treatment of various forms of pain. Methods: The plant extract, at concentrations ranging from 100 - 0.07 mg/ml, was used to determine the median lethal concentration (LC

determination of lethal dose of plant extract-[mining plant]

The lethal dose (LD 50) of the plant extract was determined by ... body weight of the methanol extract of the leaves i.p. Determination of blood ... Valeriana officinalis.Valerian and Medical … Dosage :300-500 mg of valerian root herbal extract one hour before bedtime for insomnia.

Anti-cobra venom activity of plant Andrographis paniculata ...

The ethanolic extract of plant A. paniculata significantly increases mean survival time and the protection fold, but could not protect animals from death when used alone. The higher dose, i.e., 2 g/kg was found better than that of the lower. ASV was found more effective than the plant extract. When ...

Hypoglycemic Activity of Artemisia herba-alba (Asso.) used ...

Determination of the median lethal dose (L.D 50) of the crude extract Adult male albino rats with average weight of (100-120) were used for the determination of median lethal dose (L.D 50) of the crude extract was a single oral dose through a stomach tube. The lethal dose (L.D 50) of the 70% ethyl alcohol extract of

Toxicity Studies of the Crude Aqueous Leaves Extracts of ...

The median lethal dose (LD 50) of the plant extract was determined by method of Lorke (1983) [27] using twelve [12] rats weighing between 100 – 190g. In the first phase, four rats were divided into two groups of two rats each and were treated with the aqueous leaf extract of the plant at dosages of 1600 and 2000mg/kg body weight intra ...

Acute Toxicity (Lethal Dose 50 Calculation) of Herbal Drug ...

arithmetic method of Karber [15] was used for the determination of LD50. LD50 LD100 = −∑ ×(a bn) n = total number of animal in a group. a = the difference between two successive doses of administered extract/substance. b = the average number of dead animals in two successive doses. LD100 = Lethal dose causing the death of all test ...

DETERMINATION OF MEDIAN LETHAL DOSE OF ETHANOLIC …

Packaging of Extract/Weight A spatula was used to collect the extract into a 20ml weighed beaker (pyrex), covered with a foil paper and preserved at normal room temperature. The weight of the extract was determined using a chemical balance. Determination of the Median Lethal Dose The median lethal dose of the ethanolic extract of

ACUTE TOXICITY (LD50) STUDY OF METHANOL EXTRACT OF …

Mortality recorded in intra-peritoneal Lethal Dose (LD50) determination for the extract Dose(mg/kg body weight) Methanol extract of Salacia senegalensis 0 10 4 0 100 4 0 1000 4 0 Control 4 ( 0 ) 0 = number of death, 4 = number of mice used for the test 4 Table 3.


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